The world-famous clash will see Barca host Madrid at the Estadi Olimpic Lluis Companys — their temporary home, while Camp Nou is being redeveloped.
Madrid have won all four of their league games so far this season and sit top of the LaLiga table on 12 points, with Barcelona two points behind them.
The two giants met a total of five times last season: twice in LaLiga, in the final of the Spanish Supercopa, and in the two-legged Copa del Rey semifinals.
Madrid beat Barca 3-1 in the league at the Bernabeu last October, although Barca triumphed by the same scoreline in the Supercopa final in Riyadh, Saudi Arabia, on Jan. 15.
In the Copa del Rey, Madrid came out on top, overturning a 1-0 first leg deficit with a 4-0 win at Camp Nou on April 5.
Barcelona won 2-1 in LaLiga at Camp Nou on March 19, helping them clinch the league title, eventually finishing 10 points clear of Madrid.
The two teams also met in preseason, with Barca winning 3-0 at AT&T Stadium in Arlington, Texas on July 29.
Both clubs made significant improvements to their squads this summer.
Madrid signed England midfielder Jude Bellingham — the league’s top scorer with five goals in four games — Spain international Joselu, goalkeeper Kepa Arrizabalaga, Fran García, Brahim Díaz and Arda Güler.